Here's the short version of Battle of Britain Spitfires and 100 octane fuel. +12 lb boost was obtained on Spitfire Is, Hurricanes and Defiants by operation of the
boost control cut-out and could only be employed in conjunction with 100 octane fuel. Emergency use of +12 lbs./sq.in boost was officially adopted 20 March 1940 with the release of the Air Ministry's Air Publication
A.P.1590B/J.2-W.. Below is documentation of use of emergency boost, only obtained with 100 octane fuel, by Spitfire Squadrons during the Battle of Britain: 19, 41, 54, 64, 65, 66, 72, 74, 92, 152, 222, 234, 602, 603, 609, 610, 611, 616 = 18 squadrons. I havn’t got around to checking on 266 yet. Spitfire Squadrons began converting to 100 octane and +12 boost in March 1940:

Spitfire Squadrons used it over Dunkirk in May 1940:
19 Sqdn., F/LT Brian Lane, 26 May 1940: “I gradually drew away from E/A using 12 lb. boost”

and right on through the Battle...
41 Sqdn:, F/Lt. John Webster, 28 and 29 July 1940: "I returned home at 0 feet 12 boost"
http://www.spitfireperformance.com/webster-28-7-40.jpg41 Sqdn., P/O George Bennions, 28 July 1940: “Using the emergency boost”
http://www.spitfireperformance.com/bennions-28-7-40.jpg54 Sqdn., Al Deere, 26 May 40: “I gave chase using +12 boost”
http://www.spitfireperformance.com/deere-26-5-40.jpg54 Sqdn., F/Lt George Gribble, 15 August 1940: “I dived to the attack, using 12 boost”
http://www.spitfireperformance.com/gribble-12lbs.jpg54 Sqdn., P/O Colin Gray (NZ), 25 May 1940: “I pressed the emergency boost tit”
64 Sqdn., P/O Art Donahue, 5 August 1940, “I pushed in my emergency throttle”
65 Sqdn., Jefferey Quill: “It was only shortly before the Battle of Britain that we changed over to 100 octane.”
66 Sqdn., F/O Robert Oxspring, 6 September 1940: “I shoved the throttle through the gate for emergency power.”
72 Sqdn., P/O R. D. Elliott, 9 September 1940: “with the aid of MAX Boost (12 lbs)”
http://www.spitfireperformance.com/elliott-9-9-40.jpg74 Sqdn., P/O John Freeborn, 24 May 1940: “I got away from it by using the boost cut out”.
http://www.spitfireperformance.com/freeborn-24-5-40.jpg74 Sqdn., F/Lt D. P. Kelly, 28 July 1940: “I found it necessary to use boost cutout”
92 Sqdn., Geoffrey Wellum, “Press the emergency boost override”
152 Sqdn., P/O Roger Hall, 4 September 1940: “emergency boost must not be used for more than five consecutive minutes, but now the occasion seemed to warrant the risk”
222 Sqdn., F/O D. McMullen, 15 October 1940: “I overtook E/A easily without 12 boost”
234 Sqdn., P/O Bob Doe: “Once we were in the vicinity of the enemy, I would 'pull the plug', which was the release so that we could get extra boost”
602 Sqdn., F/Lt Robert F. Boyd, 18 August 1940: “My Spitfire easily outdistanced Me 109's at 10 lbs boost 2800 r.p.m”
http://www.spitfireperformance.com/boyd-18-8-40.jpg603 Sqdn., P/O James Morton, 28 August 1940: “I was being followed so pressed the tit”
603 Sqdn., P/O Ronald Berry, 31 August 1940: “To overhaul him I had to press the emergency boost”
http://www.spitfireperformance.com/berry-31-8-40.jpg603 Sqdn., Sgt Jack Stokoe, 1 September 1940: “pushed in boost override”
http://www.spitfireperformance.com/stokoe-1-9-40.jpg603 Sqdn., F/O Brian Macnamara, 27 September 1940: “with full throttle and the red lever pressed”
609 Sqdn., P/O David Crook , 30 Sept 1940: “'pulled the plug', i.e. pushed the small handle on the throttle quadrant that cuts out the automatic boost control thus allowing one to use emergency power”.
610 Sdqn: Hawkinge, July 1940: Check the fuel bowser


611 Sdqn., 21 March 1940: “The new aircraft are one by one being converted for the use of 100 octane fuel”
http://www.spitfireperformance.com/no611-100oct.jpg616 Sqdn., F/O Hugh Dundas, 15 August 1940, “rammed the throttle 'through the gate' , to get the maximum power output, permissible for only a very limited time.”
For the expanded version see:
http://www.spitfireperformance.com/spit1vrs109e.html