Regarding special mobo or windows settings, you shouldn't need to do anything to install 4 gig. You won't actually be able to USE all 4 gig since some of the address space used for devices like the video card are mapped under the 4 gig line, essentially reducing the amount of system memory by the amount of memory reserved by the device. Every single device in your computer uses that memory, and it's all mapped under the 4 gig line.
As an example, if you have a vid card with 512 meg vid memory, you can expect to lose 512 meg of usable system memory.
This is a general rule with generic windows installations. There are ways to limit how bad this issue affects you with various different operating systems, but it's not worth it unless you KNOW you are memory limited. I think the 64 bit versions of windows don't have this problem as bad, but poor 64 bit drivers make 64 bit windows generally slower than 32 bit windows so any memory you gain is pretty much offset by the slower overall system speed and poor drivers.