I don't know about the OP, but when I tested it myself I flew the airplane manually and used manual trim control to achieve best results. You have no choice but to do it that way--the automatic settings don't work right up that high and will give you a best altitude of about 37K. Note that I did this a little over a year ago. If there's been some major revision of the Aces High P-51D flight model since then my knowledge would be out of date, but I know of no such revision.
Manifold pressures observed during climbs:
20k.......67
21k.......67
22k.......67
23k.......67
24k.......64
25k.......62
26k.......58.7
27k.......56
28k.......53.2
29k.......51
30k.......49
31k.......47
32k.......45.2
33k.......43
34k.......41
35k.......39.5
36k.......37.5
37k.......36
38k.......34
39k.......33
Here are altitudes I was able to achieve at various weights:
75% fuel, 6 guns, full ammo (combat load): 38,600
75% fuel, 6 guns, no ammo: 39,100
50% fuel, 4 guns, no ammo: 39,250
I did these tests offline and in the final test was very low on fuel by the time I reached 35K-plus.
I did speed tests as well, but seem to have deleted that file. My recollection is that speeds were generally in order, though perhaps a hair (5-7 MPH or so) optimistic for a P-51 running at only 67'' MAP.
J_A_B