Did you, by chance, have a system crash? If so, you may have been prompted to hit F1 for setup or F2 for default settings.
If I remember right, the default setting for this mobo disables the USB ports.
I am not familiar with the bios settings but you need to re-enable USB ports. Be careful as a wrong setting can turn your machine into a brick.
However, look for a setting like onboard usb support and change to enable.