G is a measure of acceleration. Metes per second squared? If you're upside down or rightside up, as long as you're stable in terms of your flight it's 1G.
Inverted or upright, if you're flying level with no sink at a steady speed I'd say that's 1G.
Now inverted 1G can still cause issues with engine pumps, lubricants, oil feeds, radiator feeds, etc. There's only 1G acting on them, but it's going in a different direction than they need it to go for them to operate properly. Kind of like how long can you last standing on your head before the blood rushes to it and you have to stop? Your body didn't break down, it didn't lose structural integrity from stresses, but the fluids are meant to pump in certain ways with and against gravity, and if you reverse that direction it can lead to problems.
Same with engines.
EDIT: Let's put it this way, P-51 guns were known to jam if fired at -1G or more. It's not that they fell apart and couldn't work... It's that they were designed to feed with gravity's help, and wouldn't function fighting against gravity.